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By Maria Lambros, Rajesh R. Patil, and Rakesh Gollen

Rajesh PatilRakesh GollenIn today’s research arena, fluorescent labeling is known to be an important aspect of various biological studies, and to date, a huge variety of chemical dyes are used extensively to label biological specimens. This in vivo imaging of both mRNAs and DNA production, localization, and dynamics is a critically important tool for live cell studies in the area of oncology to develop various cancer treatments.

For example, single cell resolution imaging has shown that tumor cells detach from tumors and, assisted by macrophages and endothelial cells, get into blood vessels and travel in the bloodstream. Dual-color fluorescent cells, with one color fluorescent protein in the nucleus and the other color fluorescent protein in the cytoplasm, allow us to evaluate the effect of the drug on the nuclear-cytoplasmic dynamics in real time, both in vivo and in vitro, and additionally evaluate cell trafficking in blood vessels and lymphatics. These imaging techniques are of paramount importance to better understand cancer and its metastasis and will help in systematic development of more effective anticancer agents.

For those who are interested in learning the advances of imaging techniques in oncology, we are hosting the symposium State-of-the-Art Imaging in Drug Development during the upcoming 2015 AAPS Annual Meeting and Exposition in Orlando. The symposium will focus on recent state-of-the-art imaging techniques along with fluorescent proteins and animal models to provide an unprecedented thrust in the development of new anticancer agents. This symposium will report recent advances in imaging at the micro and macro levels and will explain the applicability of different imaging technologies such as real-time in vivo imaging and multiphoton microscopy, to image tumors and their stroma at the cellular level, and also provide an understanding of cancer metastatic potential. We hope to see you there, and happy learning!

Maria Lambros, M.S., Ph.D., is an associate professor of pharmaceutical sciences at the Western University College of Pharmacy, and her research interests are mainly on physicochemical evaluation and formulation of pharmaceuticals, drug delivery systems including aerosols and dermatology, including studying the interaction of proteins with lipid membranes.
Rajesh R. Patil, Ph.D., is currently associate director, R&D, at Dr. Reddy’s Laboratories Inc., Princeton, NJ. His current work focusses on development of complex formulations to meet unmet therapeutic and technological needs.
Rakesh Gollen is currently pursuing his Ph.D. from Long Island University, with a major in Drug Metabolism and Pharmacokinetics, under the supervision of David Taft, Ph.D. His research focus is on the predictions of pharmacokinetic parameters in special population, using the physiologically based pharmacokinetics modeling approach.